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KMID : 0370219980420020149
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Yakhak Hoeji
1998 Volume.42 No. 2 p.149 ~ p.152
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Analysis of DA-6034 , a New Flavonoid Derivative in Biological Fluids by HPLC
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이종진/Lee JJ
손미원/유무희/장민선/김원배/이강춘/Son MW/Yoo MH/Jang MS/Kim WB/Lee KC
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Abstract
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A high performance liquid chromatographic method was developed for the determination of DA-6034 in biological fluids using internal standard. Plasma containing DA-6034 and inter nal standard was extracted by liquid-liquid extraction at an acidic pH. After evaporation of the organic layer, the drug and internal standard were reconstituted with mobile phase and injected into the column. They were separated by high performance liquid chromatography on inertsil ODS II column at 334 nm. The detection limit of DA-6034 in plasma was 0.02 mcg/ml. In this method, the range of recovery and coefficients of variation were 96-110% and 0.40-3.78%, respectively. There was no interference from endogenous substances. Urine and bile were analysed using the deproteinization method and the detection limit of DA-6034 was 1mcg/l.
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KEYWORD
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